Choose a pipeline:

From Human skeleton, England SK1964, sample Tooth (Second premolar root) ACAD12880

Extracted 2012-08-24: 0.20g of tooth powder used for extraction (http://dx.doi.org/10.1038/nature14317)

Library

Date:
2013-06-24
Type of material:
Genomic
Layout:
Paired
Type of library:
Shotgun
Construction:
DNA repair with USER, DNA library amplification by PCR using AmpliTaq Gold (http://dx.doi.org/10.1038/nature14317)
Repair method:
USER
Enrichment method:
None
Target amplified using:
2x13 cycles of PCR with AmpliTaq Gold polymerase

Sequence

Date:
2013-06-24
Sequenced at:
Wellcome Trust Sanger Institute
Sequencing method:
WGS (Whole genome shotgun sequencing)
Sequencer used:
Illumina HiSeq2500 (chemistry version 3)
Sequencing options:
Default
Output format:
fastq
Quality scale:
PHRED+33

Processing

Processed using:
AdapterRemoval/BWA/BCFTOOLS version 1.5.2/0.7.3a/1.2
Aligned to:
Hg19
Average fold coverage:
1.320x

From Human skeleton, England SK241, sample Tooth (First molar root) ACAD12881

Extracted 2012-08-24: 0.17g of tooth powder used for extraction (http://dx.doi.org/10.1038/nature14317)

Library

Date:
2013-06-24
Type of material:
Genomic
Layout:
Paired
Type of library:
Shotgun
Construction:
DNA repair with USER, DNA library amplification by PCR using AmpliTaq Gold (http://dx.doi.org/10.1038/nature14318)
Repair method:
USER
Enrichment method:
None
Target amplified using:
2x13 cycles of PCR with AmpliTaq Gold polymerase

Sequence

Date:
2013-06-24
Sequenced at:
Wellcome Trust Sanger Institute
Sequencing method:
WGS (Whole genome shotgun sequencing)
Sequencer used:
Illumina HiSeq2500 (chemistry version 3)
Sequencing options:
Default
Output format:
fastq
Quality scale:
PHRED+33

Processing

Processed using:
AdapterRemoval/BWA/BCFTOOLS version 1.5.2/0.7.3a/1.2
Aligned to:
Hg19
Average fold coverage:
4.350x

From Human skeleton, England SK5518, sample Tooth (Upper right canine root) ACAD12883

Extracted 2012-08-24: 0.20g of tooth powder used for extraction (http://dx.doi.org/10.1038/nature14317)

Library

Date:
2013-06-24
Type of material:
Genomic
Layout:
Paired
Type of library:
Shotgun
Construction:
DNA repair with USER, DNA library amplification by PCR using AmpliTaq Gold (http://dx.doi.org/10.1038/nature14320)
Repair method:
USER
Enrichment method:
None
Target amplified using:
2x13 cycles of PCR with AmpliTaq Gold polymerase

Sequence

Date:
2013-06-24
Sequenced at:
Wellcome Trust Sanger Institute
Sequencing method:
WGS (Whole genome shotgun sequencing)
Sequencer used:
Illumina HiSeq2500 (chemistry version 3)
Sequencing options:
Default
Output format:
fastq
Quality scale:
PHRED+33

Processing

Processed using:
AdapterRemoval/BWA/BCFTOOLS version 1.5.2/0.7.3a/1.2
Aligned to:
Hg19
Average fold coverage:
3.840x

From Human skeleton, England SK1231, sample Tooth (Lower third molar root) ACAD12884

Extracted 2012-08-24: 0.17g of tooth powder used for extraction (http://dx.doi.org/10.1038/nature14317)

Library

Date:
2013-06-24
Type of material:
Genomic
Layout:
Paired
Type of library:
Shotgun
Construction:
DNA repair with USER, DNA library amplification by PCR using AmpliTaq Gold (http://dx.doi.org/10.1038/nature14321)
Repair method:
USER
Enrichment method:
None
Target amplified using:
2x13 cycles of PCR with AmpliTaq Gold polymerase

Sequence

Date:
2013-06-24
Sequenced at:
Wellcome Trust Sanger Institute
Sequencing method:
WGS (Whole genome shotgun sequencing)
Sequencer used:
Illumina HiSeq2500 (chemistry version 3)
Sequencing options:
Default
Output format:
fastq
Quality scale:
PHRED+33

Processing

Processed using:
AdapterRemoval/BWA/BCFTOOLS version 1.5.2/0.7.3a/1.2
Aligned to:
Hg19
Average fold coverage:
11.830x

From Human skeleton, England 355, sample Tooth (Lower second molar root) ACAD12885

Extracted 2012-08-24: 0.14g of tooth powder used for extraction (http://dx.doi.org/10.1038/nature14317)

Library

Date:
2013-06-24
Type of material:
Genomic
Layout:
Paired
Type of library:
Shotgun
Construction:
DNA repair with USER, DNA library amplification by PCR using AmpliTaq Gold (http://dx.doi.org/10.1038/nature14322)
Repair method:
USER
Enrichment method:
None
Target amplified using:
2x13 cycles of PCR with AmpliTaq Gold polymerase

Sequence

Date:
2013-06-24
Sequenced at:
Wellcome Trust Sanger Institute
Sequencing method:
WGS (Whole genome shotgun sequencing)
Sequencer used:
Illumina HiSeq2500 (chemistry version 3)
Sequencing options:
Default
Output format:
fastq
Quality scale:
PHRED+33

Processing

Processed using:
AdapterRemoval/BWA/BCFTOOLS version 1.5.2/0.7.3a/1.2
Aligned to:
Hg19
Average fold coverage:
0.910x

Analysis

Analysed using AdapterRemoval/BWA/BCFTOOLS version 1.5.2/0.7.3a/1.2

Please cite as

Schiffels, Stephan; Haak, Wolfgang; Llamas, Bastien; Tyler-Smith, Chris; Cooper, Alan; Durbin, Richard (2016): Hinxton_WGS. Online Ancient Genome Repository. Dataset. http://dx.doi.org/10.4225/55/5779FA155A7D5

Other Studies

This dataset doesn't appear in any other studies.

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