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From Human left tibia (dorsal side of the shaft), Russia Kostenki_14, sample Bone (left tibia (dorsal side of the shaft)) Kostenki_14

Extracted: 89 to 260 mg of bone powder per extraction session (http://dx.doi.org/10.1371/journal.pone.0055950;doi:10.1038/nprot.2014.063)

Library

Type of material:
Genomic
Layout:
Paired
Type of library:
Shotgun
Construction:
(http://dx.doi.org/10.1093/bioinformatics/btt193)
Repair method:
USER
Enrichment method:
5-methylcytidine antibody
Target amplified using:
8 to 14 PCR cycles in 50 ul reactions using 5U of the AmpliTaq Gold DNA polymerase

Sequence

Sequenced at:
Danish National High-Throughput DNA Sequencing Centre, University of Copenhagen, Denmark
Sequencing method:
WGS (Whole genome shotgun sequencing)
Sequencer used:
Illumina HiSeq2500 (chemistry version 3)
Sequencing options:
Default
Output format:
fastq
Quality scale:
33

Processing

Processed using:
PALEOMIX /mapDamage/AdapterRemoval/bwa/GATK version ??/2/??/0.5.9-r26-dev/??
Aligned to:
hg19
Average fold coverage:
2.840x

Analysis

Analysed using samtools/bcftools version 1.2/1.2

Please cite as

Seguin-Orlando, Andaine; Korneliussen, Thorfinn S; Sikora, Martin; Malaspinas, Anna-Sapfo; Manica, Andrea; Moltke, Ida; Albrechtsen, Anders; Ko, Amy; Margaryan, Ashot; Moiseyev, Vyacheslav; Goebel, Ted; Westaway, Michael; Lambert, David; Khartanovich, Valeri; Wall, Jeffrey D; Nigst, Philip R; Foley, Robert A; Mirazon Lahr, Marta; Nielsen, Rasmus; Orlando, Ludovic; Willerslev, Eske (2014): Kostenki14. Online Ancient Genome Repository. Dataset. http://dx.doi.org/10.4225/55/579D55BF09508

Other Studies

This dataset doesn't appear in any other studies.

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